Web12 apr. 2024 · The expression of NLRP10 was significantly knocked down by three synthesized siRNAs (P < 0.05; Fig. 1C).Reduction of NLRP10 led to significantly reduced inflammatory cytokines (IL‐6, IL‐1β) release under stimulation with AGEs (P < 0.05; Fig. 1D).Additionally, the second siRNA was chosen for the following experiments, since it … Web15 mrt. 2024 · Mitochondrial ROS production, lysosomal rupture and mitochondrial membrane potential were assessed by flow cytometry. Briefly, to measure Mitochondrial ROS production, cells were primed with ultra-pure LPS (1 μg/ml) for 3 h in the presence of EP (5 mM), followed by treatment with ATP (5 mM,30 min) or Nigericin (10 μM,1 h).
Analysis of the Mitochondrial Membrane Potential Using the …
Web15 feb. 2011 · Many metabolic circuitries and signaling pathways involve mitochondria. Just a few examples of these include the following: oxidative phosphorylation generates … Web26 nov. 2013 · Mevalonic aciduria, a rare autosomal recessive disease, represents the most severe form of the periodic fever, known as Mevalonate Kinase Deficiency. This disease is caused by the mutation of the MVK gene, which codes for the enzyme mevalonate kinase, along the cholesterol pathway. Mevalonic aciduria patients show recurrent fever … marvins corner lunch diner
Antioxidants Free Full-Text Mito-TIPTP Increases Mitochondrial ...
THP-1 cells were treated with TSLP for 8 h, and JC-1 dye (Invitrogen, Waltham, USA) was used to measure the mitochondrial membrane potential by LSR II flow cytometry (Becton Dickinson, San Jose, CA). JC-1 dye exhibits potential-dependent accumulation in mitochondria, indicated by a … Meer weergeven THP-1 cells (American Type Culture Collection, Rockville, MD, USA) were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA). The cells were resuspended in fresh media at a concentration … Meer weergeven ChIP was performed as described previously with minor modifications [46]. Primers and probes were designed to analyze the proximal promoter regions relative to the … Meer weergeven The mitochondrial fraction was isolated from THP-1 cells using a mitochondrial isolation kit, and the activities of complex I and complex … Meer weergeven To detect mitophagy, we used the Mitophagy Detection Kit (Dojindo Molecular Technologies, Inc., Rockville, MD, USA) according to the manufacturer’s instructions. … Meer weergeven Web31 mrt. 2024 · Traba J, Kwarteng-Siaw M, Okoli TC, Li J, Huffstutler RD, Bray A, Waclawiw MA, Han K, Pelletier M, Sauve AA, Siegel RM, Sack MN. Fasting and refeeding differentially regulate NLRP3 inflammasome activation in human subjects. J Clin Invest. 2015 Nov 3;125(12):4592-600. doi: 10.1172/JCI83260. Web1 jan. 2015 · Mitochondrial ROS govern the LPS-induced pro-inflammatory response in microglia cells by regulating MAPK and NF-κB pathways Activation of microglia cells in … marvins corner diner