2024 Klf4 phase separation

Klf4 phase separation

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August undefined, 2024

WebAug 28, 2013 · KLF4, as a member of KLF family, expresses in a wide range of tissues in mammals, and plays a critical role in regulating a diverse array of cellular processes … WebDec 17, 2024 · Phase separation promotes cell fate transitions, whereas disruption of phase attenuates the transitions. The major challenge is how to effectively predict mutation residues. For complete details on the use and execution of this protocol, please refer to Wang et al. (2024). Graphical abstract Download : Download high-res image (225KB)

How Transcription Factor KLF4 Influences Gene Expression

WebDec 21, 2024 · Phase separation Germ granules are the earliest known and most studied phase-separated membrane-less organelles in germ cells during early embryonic … WebOct 7, 2024 · Phase separation is an emerging candidate mechanism that may bridge individual factors and macromolecular structures such as those mediating cell fate … horline函数

KLF4 defines the efficacy of the epidermal growth factor receptor ...

WebHere we investigate the role of DNA in nucleation of condensates, using the pioneer transcription factor KLF-4. We show that KLF-4 forms liquid-like condensates on the DNA … WebApr 14, 2024 · 63.Multivalent interactions between molecular components involved in fast endophilin mediated endocytosis drive protein phase separation. 参与快速内啡肽介导的内吞作用的分子成分之间的多价相互作用驱动蛋白质相分离。 64.Liquid-infused microstructured bioadhesives halt non-compressible hemorrhage. WebApr 13, 2024 · Many proteins undergo a process of phase separation that leads to the formation of biomolecular condensates. It has been observed that some of these proteins form dense droplets of sizeable dimensions already below their critical concentration for phase separation. To understand this phenomenon, which is not readily compatible with … losing my mind polo g lyrics

New insight into the significance of KLF4 PARylation in …

Surface condensation of a pioneer transcription factor on DNA

WebOct 19, 2016 · The newt RPE has also been studied with respect to the molecular mechanism of cell entry into the S-phase after RPE separation from the NR [62,63]. The authors of the first study analyzed an activity of MEK-ERK signaling in RPE just after NR removal from the newt eye and found it activated within 30 min after retinectomy. ... Mitf, … WebFeb 25, 2013 · We have identified the zinc-finger transcription factor Kruppel-like factor 4 (Klf4) among the transcription factors that are significantly downregulated in their expression during epithelial-mesenchymal transition (EMT) in mammary epithelial cells and in breast cancer cells. Loss and gain of function experiments demonstrate that the down … losing my mind meme piggyWebAug 17, 2024 · Liquid-liquid phase separation (LLPS) is an important organizing principle for biomolecular condensation and chromosome compartmentalization. However, while many proteins have been reported to undergo LLPS, quantitative and global analysis of chromatin LLPS property remains absent. Results losing my mind one kid at a time svg

"WebJun 18, 2024 · KLF4 represses transcription of the EGFR gene, leading to reduced levels of total EGFR, its activated/phosphorylated form (pEGFR), and its downstream signaling intermediates. Moreover, KLF4 suppression of EGFR is a necessary intermediary step for KLF4 to inhibit aggressive TNBC phenotypes. " - Klf4 phase separation

Klf4 phase separation

How Transcription Factor KLF4 Influences Gene Expression

WebApr 5, 2024 · Now, in a new study, scientists have answered that question, showing that viral proteins use a process called phase separation to coordinate production of viral progeny. WebDec 26, 2024 · 2.1. LH Regulates the Expression of Klf4, Bcl-2, and Cell Cycle Genes in Preovulatory GCs. To investigate changes in the expression of Klf4 and the genes regulating apoptosis and the cell cycle in preovulatory GCs in the early phase of LH exposure, cells were cultured in the presence of LH (0, 100, 200 ng/mL) for 45 min, and real-time PCR …

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WebDec 26, 2024 · In agreement with this, Klf4 overexpression reduced cell viability, increased the fraction of apoptotic cells, and arrested cell cycle progression in G1 phase. We conclude that Klf4 increases the susceptibility of preovulatory GCs to apoptosis by down-regulating Bcl-2, and promotes LH-induced cell cycle exit. It appears to be a key regulator ...

WebKLF4 belongs to the Kruppel-like factor (KLF) family, a group of conserved zinc finger TFs that regulates essential biological processes such as proliferation and development. Reflecting pioneer factor activity, KLF4 uses two out of its three zinc fingers to target a GC-rich hexameric motif enriched within nucleosomal targets22 ( Fig. 7.2 C). WebAug 28, 2013 · These studies suggest that KLF4 is a critical factor in regulating entry of the cells into the mitotic phase. Finally, KLF4 was found both necessary and sufficient in preventing centrosome amplification ... Characterization of different KLF4 isoforms and/or separation of distinct KLF4 expressing cells will be necessary for dissecting specific ...

WebSep 25, 2024 · We demonstrate that Klf4 forms microphases that are enabled by interaction with the DNA surface. This sets their typical size and allows them to form below the … WebMar 23, 2024 · The evident changes observed in cell morphology together with the downregulation of the naïve pluripotency markers Nanog, Klf4 and Esrrb and the increase of the mRNA levels of primed...

WebApr 12, 2024 · Hydrocyclones are widely used in the separation of two-phase immiscible mixtures. In various application scenarios, three-phase hydrocyclones (3PH) are often urgently needed. In this work, the typical 3PHs are classified into six categories according to their structural characteristics. A comprehensive analysis of their structural feature ...

WebApr 4, 2024 · KLF4 is a therapeutically tractable brake on fibroblast activation that promotes resolution of pulmonary fibrosis. Epithelial-mesenchymal reprogramming by KLF4-regulated Rictor expression contributes to metastasis of non-small cell lung cancer cells. losing my mind kdrew lyricsWebSep 22, 2024 · KLF4 is thought to induce long-range pluripotency contacts through condensation at promoters of pluripotency factors (Sharma et al. 2024 ). However, KLF4 condensation depends on KLF4-DNA... horlimerWebFeb 3, 2024 · We show that Klf4 can phase separate on its own at high concentrations, but at low concentrations, Klf4 only forms condensates on DNA. Using optical tweezers, we … horlinaWebApr 17, 2024 · The relationship between the molecular weight of PVDF and its distribution, phase separation, crystallization behavior and spinning process has been systematically studied. The effects of three factors on the microstructure and properties of the PVDF membrane have been analyzed. The flow behaviors of the PVDF/diluent and PVDF melt … hor liftWebFor this ratio, we observe four categories of ligand-modulated phase behaviors of scaffolds: 1) Ligand binding abolishes scaffold phase separation (phase diagrams with red bounding boxes in Fig. 2 C ); 2) ligand binding destabilizes scaffold phase separation (phase diagrams with blue bounding boxes in Fig. 2 C ); 3) ligand binding does not impact … losing my mind kdrewWebHere we investigate the role of DNA in nucleation of condensates, using the pioneer transcription factor KLF-4. We show that KLF-4 forms liquid-like condensates on the DNA surface at physiological concentrations, below the one required for Klf4 phase separation. losing my mind sheet music pdfWebApr 11, 2024 · Phase separation of biomolecules occur to form membraneless compartments in response to extra- or intra-cellular stimuli. Recently, phase separation has been identified in different immune signaling pathways, of which cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling is an important innate immune … horline什么意思