WebApr 12, 2024 · Briefly, the bEnd.3 cells were seeded and cultured in a 24-well cell culture plate. After treating the cells under hypoxia condition, the cells were incubated with DCFH-DA (10 mM, diluted in serum-free medium) and Hoechst (94403, Sigma-Aldrich; 1:1000; diluted in 5% BSA) for 20 min at 37°C in the dark. Web2 days ago · Stained cells were visualized using a Nikon A1R Confocal Microscope (SickKids Imaging Facility, Toronto) with excitation wavelengths of 405 and 568 to detect signals from Hoechst 33342 and CSRBnps respectively. The laser power of CSRBnps excitation wavelength was kept constant for image acquisition of all samples.
Hoechst stain - Wikipedia
WebOct 25, 2024 · Hoechst 33258 staining was used to observe the effect of E. officinalis on NHDF cell apoptosis 72 h after exposure to 144 mJ/cm 2 of UVB ... These cells were placed away from light for 30 min at room temperature and then inspected using flow cytometry with excitation at 490 nm and emission at 525 nm (FACSCaliburTM; Becton-Dickinson, … WebHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed … fact or bluff statements
DAPI (4
WebFor Hoechst 33342-DNA the mode of excitation was a mixture of two- and three-photon excitation to the longest excitation wavelength of 910 nm, and we were unable to observe … WebHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the SP strategy, is detected at two emission wavelengths after UV excitation: Hoechst Red (630–650 nM) and Hoechst Blue (405–450 nM). WebFor imaging Hoechst 33342 staining, a 405 nm laser was used for excitation (solid-state laser rated power = 100 mW) together with a blue 430–470 nm emission filter. Live/dead cell analysis was performed using IN Carta analysis software128 (GE Healthcare). does the oculus link cable charge the headset