Clonal seeds in hybrid rice using crispr/cas9
WebRecently, CRISPR-Cas9-based genome editing system has been established for banana in a few laboratories. Here, we describe the procedures for generation of genome-edited events of banana, detection of targeted and potential off-target mutations, and phenotyping for important traits such as disease resistance. WebJan 1, 2024 · Hybrid rice is cultivated in more than 40 countries and occupies ~60% of the rice-production area in China, giving an average yield advantage of 10–20% over conventional inbred cultivars ( Yuan, 2014 ), and hybrid wheat was cultivated on 560,000 ha in Europe in the 2024–2024 growing season ( Gupta et al., 2024 ).
Clonal seeds in hybrid rice using crispr/cas9
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WebApr 11, 2024 · Rice seeds were sterilized with 75% ethanol for 1 min and 2.5% sodium hypochlorite for 15 min in sequence, followed by 3–5 times rinses with sterile distilled water. Then the seeds were placed on 1/2 Murashige and Skoog (MS) medium with agar for germination. The hydroponic culture was the Yoshida nutrient solution (YNS) (Impa et al. … WebJP2024037006A JP2024004286A JP2024004286A JP2024037006A JP 2024037006 A JP2024037006 A JP 2024037006A JP 2024004286 A JP2024004286 A JP …
WebCRISPR–Cas9 edited mutations in BBM1, BBM2 and BBM3 in rice a, DNA sequences of mutations in bbm1/bbm1 bbm3/bbm3 plants. b, DNA sequences of mutations in bbm2/bbm2 bbm3/bbm3 plants. a and b... WebMar 9, 2024 · We used CRISPR-Cas9 to precisely delete the Waxy1 gene in multiple elite lines. We conducted molecular characterization of CRISPR-waxy plants: confirming the expected deletion, evaluating...
WebJan 9, 2024 · Researchers also edited four genes, REC8, PAIR1, OSD1 and MTL, in hybrid rice for clonal reproduction of F 1 rice hybrids through seeds ( Wang et al., 2024 ). Therefore, the extensive usage of this system provides new scientific guidance for adopting advanced techniques to develop elite rice varieties. WebJan 4, 2024 · Clonal propagation through seeds would enable self-propagation of F1 hybrids. Here we report a strategy to enable clonal reproduction of F1 rice hybrids through seeds. We fixed the heterozygosity of F1 hybrid rice by multiplex CRISPR–Cas9 genome editing of the REC8, PAIR1 and OSD1 meiotic genes to produce… Expand View on …
WebSep 24, 2024 · Processing samples to extract DNA of sufficient purity for PCR is often a limiting step. We have developed a simple 96-well plate-based high-throughput DNA …
WebSep 2, 2024 · By simultaneous editing of these four endogenous genes in hybrid rice using the CRISPR/Cas9 system, plants that are able to propagate clonally through seeds were obtained. The quadruple aop ... In contrast, techniques to distinguish clonal seeds or plants among offspring could be developed to further expand the application of the Fix strategy ... man regrows finger tipWeb高影响力文章. 国外数据库各学科高被引论文TOP5. 数据来源：Web of Science 文献出版时间：2013.1—2014.11 检索时间：2014.12.1 kotor 2 command codesWebAbstract Obtaining photoperiod-sensitive genic male sterility (PGMS) lines is one of the most important steps in two-line hybrid rice breeding. Traditionally, such lines were screened and developed with a classic rice breeding system under both … man regrows armWebMar 2, 2024 · As many crops are sown as hybrids to harness hybrid vigor, male-sterile lines have been widely employed during seed production; genome editing now makes it possible to efficiently and easily sort sterile line and maintainer line seeds in maize ( Zea mays ), as we previously reported ( Qi et al., 2024b ). kotor 2 command listWebSince their first adaptation for plant genome editing, clustered regularly interspaced short palindromic repeats/CRISPR-associated system nucleases and tools have revolutionized the field. While early approaches focused on targeted mutagenesis that relies on mutagenic repair of induced double-strand breaks, newly developed tools now enable the precise … man regressed into babyWebJul 26, 2024 · Recently, a study reported generation of clonal seeds in rice by simultaneous engineering of edited meiotic genes (rec8, pair1 and osd1) with disrupted matrilineal (matl/ also known as not... man reincarnated as a goblinWebSince their first adaptation for plant genome editing, clustered regularly interspaced short palindromic repeats/CRISPR-associated system nucleases and tools have revolutionized … man rejects first penis transplant